The Bioptechs Delta T™ Culture Dish System is the only culture dish that provides heat to the contents of the dish without heating the stage of the microscope. It uses number 1.5 thickness cover glass bottomed dishes that have an electrically conductive, optically transparent coating of ITO on the underside. The coating is electrically powered to directly warm the contents of the dish. This is an exclusive technology to Bioptechs and provides features and capabilities no other system can match. An intelligent feedback loop passes an electrical current through a thin-film coating on the underside surface of the glass substrate on which the cells are grown. Heat is applied directly to the cells without the inefficiencies associated with peripheral heating by traditional peripherally heated culture dish warmers. The controller features a real-time temperature display and fast learning curve to compensate for cooling due to surface evaporation while responding to temperature changes due to perfusion. There is also an alarmed protection circuit to safeguard the cells and an internal reference for the user adjustable calibration. The standard controller has a temperature range of ambient to 50° C. Extended ranges are available upon request. Accessories to close the dish and provide a closed chamber or items which accommodate a wide variety of cell types are available on the accessories page.
Delta T dishes have 35mm O.D. and a 23mm central aperture. The peripheral region of the dish is tapered to reduce the dead-volume and the height of the dish is 6mm to allow better access for micro-injection and micromanipulation. The dishes are a hybrid of polystyrene plastic and Desag 263 glass. The outer structure of the dish is available in opaque black or clear and come with a clear 0.5mm or no.1.5 glass coverslip bottom bonded to it.
Bioptechs Delta T vs. Traditional Stage Heating
Accurate temperature control
Low dead volume
Superior optical image
The thermograph on the left shows the efficiency, accuracy and uniformity of the Delta T™ system. Notice the temperature of the stage adapter. It is nearly the same temperature as the room temperature background. The dotted oval shows where the edge of the stage adapter is in visible light. Only the specimen and media are heated. Power consumption is 0.9 watts because heat is only applied to the specimen area. There is no heat transmitted to the stage. Therefore, it remains “Z” axis stable. This is a sharp contrast to traditional peripheral heating methods (shown below right), and clearly superior.
The thermograph above on the right indicates the disadvantage of peripheral heating. This is a thermal image of a 50mm culture dish in the center of a 100mm diameter uniformly heated, 3mm thick, aluminum plate with a 25 mm hole in the center. This image was acquired after 20 minutes of equilibration. Note the high temperatures of nearly 60° C, that it takes to reach 37° C in the specimen area. In this case heat that is not beneficial to the specimen is sunk into the stage causing Z-axis instability, not to mention the non-uniform temperature of the specimen area.