FCS4 Flow Chamber No Heat

Ergonomically designed parallel plate non-heated flow cell chamber. High N.A. compatible, microscopic flow cell with a user definable specimen area for inverted microscopes. Simple no tools assembly.  Includes: FCS4 Chamber, stage adapter not included.

$2,880.00

SKU 060319-4-0308-NH Category Tag

Overview

The FCS4® Flow Chamber is a versatile, easy to use solution for sample isolation with perfusion flow control that is compatible with all forms of microscopy.    The FCS4 Flow Chamber has all the same features as the FCS2 Flow Chamber with a new ergonomic design for a simplified assembly and adjustable compression. For a more detailed information about the system Click Here.

  1. FCS4 Top
  2. FCS4 Base
  3. Pressure Plate

* Requires: gaskets, coverslips, stage adapter, and micro-aquaduct slides

Features

  • Ion concentration studies (calcium, chloride, magnesium, etc.)
  • Metabolic studies
  • Cytotoxicity & apoptosis assays
  • Biochemical mechanism studies
  • Drug response studies
  • FISH with chemical (not heat dependent) hybridization
  • SeqFISH with chemical (not heat dependent) hybridization
  • MERFISH with chemical (not heat dependent) hybridization
  • Particle tracking
  • Protein trafficking
  • Vesicle trafficking
  • Membrane transport studies
  • Protein interaction
  • Laser-scanning confocal
  • Resonant scanning laser-scanning confocal
  • Two-photon & multi-photon confocal
  • SPIM
  • STED
  • SIM FRET & BRET
  • STORM
  • FLIM
  • TIRF
  • FRAP
  • Spinning disk
  • Widefield
  • Transmitted light
  • DIC
  • Phase contrast
  • Super resolution
  • And more…

Definable flow characteristics

  • Complete control of volume within optical cavity
  • Save on expensive reagents
  • Adjustable separation between optical surfaces
  • Laminar or customizable flow
  • User definable shear force

Advanced imaging compatibility

  • Compatible with all modes of microscopy 
  • Adapts to all brands of microscopes
  • ideal for high resolution imaging
  • Accommodates adherent cells, tissue, or suspended specimens

Ergonomic design

  • Self aligning assembly
  • No tools twist assembly
  • Easy to read dial for compression
  • Retro-compatible with all FCS2 Stage adapters

Chambering your sample has never been so easy

  1. FCS4 Chamber Top – Easy to use ergonomic twist on top with compression dial for sealing cell chamber. 316 Stainless steel and fully autoclavable.
  2. Perfusion Pressure Plate – Spacer machined with 316 stainless steel and perfusion tubes perfusion tubes. Fully autoclavable.
  3. Perfusion Tubes – 316 Stainless steel perfusion tubes permanently welded to the Perfusion Pressure Plate to interface perfusion tubing. 
  4. Upper Gasket – Applies pressure to ensure seal on chamber and prevent leakage and allows for perfusion.
  5. Micro-Aqueduct SlideAn optical surface which integrates perfusion, high-volume laminar flow, and koehler Illumination.  
    Fully autoclavable.
  6. Lower Gasket – This gasket can have any internal geometry you desire and comes in standard thicknesses from .1mm to 1mm. This allows you to define the volume and flow characteristics of the chamber.
  7. 40mm CoverslipSurface where your cells are grown. German Desag 263 low alkaline glass, fully autoclavable or alcohol for sterilization. 
  8. Self- Aligning Base – 316 Stainless steel, autoclavable precision base. Designed to assure parallel uniform closure, consistent stack alignment, eliminate leaks, and broken coverslips.

Perfusion Flow

A fluid pathway is formed by separating the Microaqueduct Slide from the coverslip containing cells with a single silicone gasket. This gasket can be any thickness from 50 micron to 1mm and any lateral geometry you choose or create.  Fluid access to this flow channel is made through two 14-gauge needle stock tubes protruding from the sides of the chamber top. These tubes provide fluid connection to two perfusion holes in the Microaqueduct Slide that interface two “T” shaped grooves cut into the inner surface of the Microaqueduct Slide. The “T” groove allows the media to seek the path of least resistance and become nearly laminar before flowing across the cells. This technique eliminates the need for the metal perfusion ring and additional gaskets, which are the limiting factors, required by most conventional chambers. 

For more information on the system check out the system page or feel free to contact us for immediate service.