Bioptechs has partnered with Fluigent to create the most precise solution for high quality multiplexed imaging.
Precision flow cell imaging system
Aria is an integrated microfluidic system to automate the delivery of up to 10 different fluids to one chip or perfusion chamber. It can perform any perfusion protocol. The system is easy to use, compact, and can interact with TTL enabled microscopy systems. It also allows for “stop flow” conditions for studies requiring longer imaging times. Aria automates sequential injection operations that have typically been performed manually and provides more reproducible results with minimum cross contamination.
Aria covers any applications as the volumes injected range from 40µL to hundreds of mL delivered over several days.
To minimize carryover between successive solutions, the tubing can be cleared.
The user is informed of the delivery time of each solution thanks to Aria’s intelligent software. The software also notifies the user of the minimum volume required in each reservoir to run their protocol.
Aria comes with an intuitive software that enables to design protocols in few clicks.
Incubation time, flow rate, volume dispensed, are all parameters that can be easily set by the operator for each step of his protocol.
Protocols are recorded and can be shared among users. Flow rate and pressure are recorded for each experiment.
Aria drastically reduces variability down to 0,5% between experiments compared to 5,1% intra-operator variability and 8,1% inter-operator variability using a pipette.
Aria can synchronize with various microscopes through the use of TTL signaling. Aria can send and receive TTL signals for example to start an imaging cycle, or to resume the Aria protocol when an imaging cycle is completed.
Aria’s software comes with a dark mode to work in an imaging room. Aria is equipped with LED’S to facilitate use in dark areas.
Applications like DNA hybridization, high resolution spatial transcriptomics, DNA paint, or tissue profiling require high resolution microscopy as they target nanometric structures inside the cells.
For each of these applications, the sample is successively incubated with specific probes to bind to a target inside the cells. Between various probes, a sequence of washing steps is performed to remove the previous probe and avoid contamination with the consecutive one. The power of this technique compared to traditional immunolabeling is that the number of targets per sample is not limited to the four traditional colours of the microscope as DNA can be easily detached from their target as opposed to antibody that remain strongly attached when bounded.
Manually changing solutions from washing buffers to probes on the microscope comes with some challenges:
In addition, successive solution replacement can be long (up to 4-5 days for an experiment), tedious and requires the physical presence of the scientist.
Aria is the only instrument that can automate the delivery of up to 10 different solutions to a flow cell. Aria is the perfect compromise between manual pipetting and all-in-one system that are dedicated to one specific application, integrate a microscope, specific chip type and a given set of solutions: Any protocol with multiple solution delivery can be automated, saving the scientist both time and reducing variability between experiments compared to manual procedures.
If you are interested in learning more about ARIA from Fluigent, Bioptechs Flow Cells, or would like a quote please contact us below.