The Focht Chamber System 2 (FCS2®) is a closed system, live-cell micro-observation chamber that utilized several patented technologies that offer several exclusive advantages over other chambers. It provides user definable perfusion capability, andd is fully compatible with all modes of microscopy. It is also the only chamber to combine high-volume laminar flow perfusion rates with Koehler illumination without the need for an air curtain. This is accomplished by the development of micro aqueduct perfusion. A method of introducing media into a fluid optical cavity where the separation of optical surfaces and flow geometry is definable by the user. Therefore, the chamber is adaptable to the protocol instead of having to adapt the protocol to the chamber.
A fluid pathway is formed by separating the Microaqueduct Slide from the coverslip containing cells with a single silicone gasket. This gasket can be any thickness from 50 micron to 1mm and any lateral geometry you choose or create. This arrangement allows the user to define the flow characteristics. Therefore, you are not limited by the geometry of the optical cavity. Instead you select or create it! Fluid access to this flow channel is made through two 14-gauge needle stock tubes protruding from the sides of the chamber top. These tubes provide fluid connection to two perfusion holes in the Microaqueduct Slide that interface two “T” shaped grooves cut into the inner surface of the Microaqueduct Slide. The “T” groove allows the media to seek the path of least resistance and become nearly laminar before flowing across the cells. This technique eliminates the need for the metal perfusion ring and additional gaskets, which are the limiting factors, required by most conventional chambers.
The flow cell is easily modified with a variety of gasket geometries (see additional gaskets above for a few samples), and a gasket thicknesses ranging from 0.1mm to 1mm. If the entire scope is in an environmental enclosure or, doesn’t need temperature control, the FCS2 is available in the following configuration and provides a substantial savings without the temperature control: