The Delta T Tissue Slice Adapter is an accessory designed to support a slice of natural tissue such as bowel, trachea, skin, or other natural tissue at the correct distance relative to the coverslip in the Delta T Dish. The specimen is attached to an aperture so that the specimen is constrained to being flat on a carrier that is Z-axis translatable. This allows the specimen to be adjusted in the Z-axis plane relative to the focal range of the objective. The Delta T System accommodates the fluid, thermal, and optical environment. Therefore, all that needs to be done is to define the tissue on a plane parallel to the coverslip and adjust the position the specimen relative to the objective’s plane of focus.