When imaging cells in any structure such as a chamber having a coverslip as an observation surface, one must appreciate the fact that any rapid changes in flow rate will translate to micro-dynamic changes in pressure within the dish causing the coverslip to react like a diaphragm forcing the specimen to go out of focus with each step.
To eliminate this problem at the lower flow rates and significantly reduce it at higher flow rates, Bioptechs recommends the Instech Micro-Perfusion Pump for use with all its micro-observation systems. This Micro-Perfusion Pump is a miniature, dual channel or single channel, full-featured peristaltic pump designed specifically for low-flow rates. Unlike most peristaltic pumps that are driven by stepper motors, the Micro-Perfusion Pump is driven by a tachometer regulated, multi-stage DC gear motor. This assures a smooth analog rotation of the roller spindle, free of instantaneous steps. It is regulated by either the internal control circuitry adjustable from 0.8 µL/min to 18 ml/min (dependent on pump model). The pump comes with a 15 volt AC adapter and also contains an internal 9 volt battery which can function as the primary power supply if needed. The pump tubes are available in silicone and C-Flex. They are terminated with a 1/16″ tubing barb. The base of the pump is threaded for easy mounting to a stand or fixture near the microscope. An optional rod mounting clamp is available for mounting to a 1/2″ rod. To prevent a culture dish from overflowing, the dual tube set has two different inside diameters. Therefore, you can simply apply media to the dish at a rate slower than it is aspirated away. To control the level in the dish, simply adjust the height of the aspiration tube.
- Usable as either single or dual channel
- Precise speed setting feature
- Flow rates of 0.4 µL/min to 18 ml/min (pump dependent)
- Compatible with 1/16″ tubing
- Universal 9VDC adapter that accepts an input of 100 – 240VAC. Power supply has plugs for US, Europe, UK, and Australia
- 9V battery backup
- Can be operated on internal battery if desired
Tubing sizes and flow rates for single tube sets:
|Tubing I.D.||Ultra Low Flow||Very Low Flow||Low Flow||High Flow|
|0.015″||0.2 – 2.2 µL/min||0.8 – 7.5 µL/min||8 – 83 µL/min||0.07 – 0.7 ml/min|
|0.020″||0.4 – 4.6 µL/min||1.6 – 16 µL/min||18 – 180 µL/min||0.15 – 1.5 ml/min|
|0.031″||0.9 – 8.7 µL/min||3 – 30 µL/min||35 – 350 µL/min||0.3 – 2.8 ml/min|
|0.062″||3 – 30 µL/min||10 – 100 µL/min||125 – 1250 µL/min||0.9 – 9 ml/min|
|0.093″||6 – 58 µL/min||20 – 200 µL/min||240 – 2400 µL/min||1.8 – 18 ml/min|
Tubing sizes and flow rates for double tube sets:
|Tubing I.D.||Part # (C-Flex) Single Tube Set||Part # (C-Flex) Double Tube Set|
* DUAL TUBE SET WARNING: Dual channel tube sets, particularly the .062 size, place more stress on the pump motor than do single channel tube sets, which will shorten the life of the pump. Pumps with spur gearheads (Low Flow, Very Low Flow and Ultra Low Flow) are more susceptible to this stress than are pumps with planetary gearheads (High Flow). In addition, dual .062 tube sets make contact with the sides of the pump rotor, which will cause those tube sets to wear faster than other sizes.