Perfusion Pumps

When imaging cells in any structure such as a chamber having a coverslip as an observation surface, one must appreciate the fact that any rapid changes in flow rate will translate to micro-dynamic changes in pressure within the dish causing the coverslip to react like a diaphragm forcing the specimen to go out of focus with each step.

To eliminate this problem at the lower flow rates and significantly reduce it at higher flow rates, Bioptechs recommends the Instech Micro-Perfusion Pump for use with all its micro-observation systems. This Micro-Perfusion Pump is a miniature, dual channel or single channel, full-featured peristaltic pump designed specifically for low-flow rates. Unlike most peristaltic pumps that are driven by stepper motors, the Micro-Perfusion Pump is driven by a tachometer regulated, multi-stage DC gear motor. This assures a smooth analog rotation of the roller spindle, free of instantaneous steps. It is regulated by either the internal control circuitry adjustable from 0.8 µL/min to 18 ml/min (dependent on pump model). The pump comes with a 15 volt AC adapter and also contains an internal 9 volt battery which can function as the primary power supply if needed.  The pump tubes are available in silicone and C-Flex. They are terminated with a 1/16″ tubing barb. The base of the pump is threaded for easy mounting to a stand or fixture near the microscope. An optional rod mounting clamp is available for mounting to a 1/2″ rod. To prevent a culture dish from overflowing, the dual tube set has two different inside diameters. Therefore, you can simply apply media to the dish at a rate slower than it is aspirated away. To control the level in the dish, simply adjust the height of the aspiration tube.